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- Title
OsDSR-1, a calmodulin-like gene, improves drought tolerance through scavenging of reactive oxygen species in rice ( Oryza sativa L.).
- Authors
Xia, Xinjie; Yin, Xuming; Huang, Lifang; Wang, Manling; Cui, Yanchun
- Abstract
Calmodulin-like (CML) genes regulate plant growth, development, and responses to abiotic stresses such as salinity and drought. Many genes encoding CML proteins have been identified from rice ( Oryza sativa), but their functions remain largely unknown. Our characterization of one putative CML gene, OsDSR-1 ( O. sativa Drought Stress Response-1), showed that its protein binds Ca and displays Ca-dependent conformational changes. In contrast to wild-type (WT) and OsDSR-1-RNA interference ( OsDSR-1-Ri) plants, transgenic rice plants that overexpress OsDSR-1 were significantly more drought tolerant and had increased sensitivity to abscisic acid. Furthermore, their concentrations of free proline and soluble sugars and the activities of reactive oxygen species-scavenging enzymes as well as the transcript levels of many ROS-scavenging and stress-related genes were significantly enhanced under drought stress. Much less hydrogen peroxide and malondialdehyde accumulated in OsDSR-1-overexpressing ( OsDSR-1-OE) plants than in either the Ri or WT plants. All of these results suggest that OsDSR-1 plays important roles in conferring tolerance to drought in rice by decreasing the occurrence of oxidative damage.
- Subjects
ABSCISIC acid; CALMODULIN genetics; CALCIUM-binding protein genetics; DROUGHT tolerance; RICE; REACTIVE oxygen species
- Publication
Molecular Breeding, 2017, Vol 37, Issue 6, p1
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-017-0668-y