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- Title
Use of small interfering ribonucleic acids to inhibit the adipogenic effect of alcohol on human bone marrow-derived mesenchymal cells.
- Authors
Huang, Qiang; Zhang, Hui; Pei, Fu-xing; Chen, Zhi-yu; Wang, Guang-lin; Shen, Bin; Yang, Jing; Zhou, Zong-ke; Kong, Qing-quan
- Abstract
This study tested the potential of small interfering RNAs (siRNA) targeting human peroxisome proliferator activated receptor gamma (PPARγ) to repress the adipogenic effect of alcohol on human bone marrow-derived mesenchymal cells (hBMSCs). hBMSCs were cultured from hip replacement surgery patients ( n = 10). PPARγ-siRNA was transiently transfected into hBMSCs cultured in ostogenic media containing 50 mM alcohol by using a liposome-based strategy. Oil red O staining was used to test the development of differentiated adipocytes, and Alizarin red staining was used to test mineral deposition. Marker genes of adipogenesis (PPARγ2 and aP2) and osteogenesis (Osf2/Cbfa1) were examined through real time RT-PCR and Western blot, respectively. Collagen type I, alkaline phosphatase and osteocalcin protein synthesis of cultures were also assayed. Data were presented as mean ± SD. Differences between the means of the treatment groups were determined with ANOVA. PPARγ-siRNA transfection resulted in significantly lower adipocyte number, increased matrix mineralisation, repressed adipogenic gene markers, up-regulated osteogenic gene marker and bone matrix protein synthesis in the PPARγ-siRNA group compared to controls ( P < 0.05). PPARγ-siRNA is a useful strategy to inhibit the adipogenic effect and the osteogenic repression of alcohol on hBMSCs. This may be a novel therapeutic intervention for osteopenic disorders in alcoholism and other conditions.
- Subjects
SMALL interfering RNA; PHYSIOLOGICAL effects of alcohol; MESENCHYMAL stem cells; TOTAL hip replacement; FAT cells; ADIPOGENESIS; BONE growth; PEROXISOME proliferator-activated receptors; PATIENTS
- Publication
International Orthopaedics, 2010, Vol 34, Issue 7, p1059
- ISSN
0341-2695
- Publication type
Article
- DOI
10.1007/s00264-009-0914-y