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- Title
Evaluation of Tanshinone IIA Developmental Toxicity in Zebrafish Embryos.
- Authors
Tao Wang; Chengxi Wang; Qiong Wu; Kangdi Zheng; Jiaojiao Chen; Yutao Lan; Yao Qin; Wenjie Mei; Baoguo Wang
- Abstract
Tanshinone IIA (Tan-IIA) is derived from the dried roots of Salvia miltiorrhiza Bunge, a traditional Chinese medicine. Although Salvia miltiorrhiza has been applied for many years, the toxicity of the mono-constituent of Salvia miltiorrhiza, tanshinone IIA, is still understudied. This study evaluated the cardiotoxicity and developmental malformations of Tan-IIA by using zebrafish normal embryos and dechorionated embryos. After treatment with Tan-IIA in different concentrations for four-day periods, obvious pericardial edema, spinal curvature, and even missing tails were observed in zebrafish embryos. The LC50 values in the dechorionated embryo group at 72 h post-fertilization (hpf) and 96 hpf were 18.5 μM and 12.8 μM, respectively, and the teratogenicity was manifested at a concentration of about 1 μM. The main endpoints of teratogenicity were scoliosis, malformation of tail, and pericardium edema. Our findings displayed the potential cardiotoxicity and severe impact on the abnormal development of Tan-IIA in zebrafish embryo at high concentrations, which may help avoid the risk of its clinical application.
- Subjects
ZEBRA danio embryos; CARDIOTOXICITY; SALVIA miltiorrhiza; SCOLIOSIS; TERATOGENICITY testing; ABNORMALITIES in animals; FISHES
- Publication
Molecules, 2017, Vol 22, Issue 4, p660
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules22040660