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- Title
Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities.
- Authors
Wei-Min Zhang; Wu-Yang Huang; Wen-Xue Chen; Lin Han; Hai-De Zhang
- Abstract
Polyphenols are functional compounds in plants, which possess many bioactivities beneficial for humans. The aim of this study was to establish a highly efficient method for extracting polyphenol compounds from areca seeds and further to identify polyphenols and antioxidant properties of the seeds. A quadratic general rotary unitized design was used to determine the optimal extraction process. The polyphenols were identified using LC-TOF-MS. By comparison with ascorbic acid (Vc), the antioxidant activities of the ethanol extracts were evaluated using three complementary in vitro assays: inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, hydroxyl radical-scavenging activity, and reducing ability. The two major polyphenols obtained were epicatechin and syringic acid. The ethanol extracts of areca seeds showed significantly greater antioxidant activity (p < 0.05) than Vc using the DPPH and reducing power assay, but lower ability (p < 0.05) using the hydroxyl radical assay. The results indicate that the areca seed is an excellent food material with potential antioxidant properties.
- Subjects
EXTRACTION (Chemistry); POLYPHENOLS; ANTIOXIDANTS; VITAMIN C; HYDROXYL group; THERAPEUTIC use of plant extracts
- Publication
Molecules, 2014, Vol 19, Issue 10, p16416
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules191016416