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- Title
Productive HIV-1 infection is enriched in CD4CD8 double negative (DN) T cells at pleural sites of dual infection with HIV and Mycobacterium tuberculosis.
- Authors
Meng, Qinglai; Canaday, David; McDonald, David; Mayanja-Kizza, Harriet; Baseke, Joy; Toossi, Zahra
- Abstract
A higher human immunodeficiency virus 1 (HIV-1) viral load at pleural sites infected with Mycobacterium tuberculosis (MTB) than in peripheral blood has been documented. However, the cellular source of productive HIV infection in HIV-1/MTB-coinfected pleural fluid mononuclear cells (PFMCs) remains unclear. In this study, we observed significant quantities of HIV-1 p24 lymphocytes in PFMCs, but not in peripheral blood mononuclear cells (PBMCs). HIV-1 p24 lymphocytes were mostly enriched in DN T cells. Intracellular CD4 expression was detectable in HIV-1 p24 DN T cells. HIV-1 p24 DN T cells showed lower surface expression of human leukocyte antigen (HLA)-ABC and tetherin than did HIV-1 p24 CD4 T cells. Upon in vitro infection of PFMC CD4 T cells from TB mono-infected subjects, Nef- and/or Vpu-deleted HIV mutants showed lower generation of HIV-1 p24 DN T cells than the wild-type virus. These data indicate that productively HIV-1-infected DN T cells, generated through down-modulation of surface CD4, likely by HIV-1 Nef and Vpu, are the predominant source of HIV-1 at pleural sites of HIV/MTB coinfection.
- Subjects
HIV infections; CD4 antigen; CD8 antigen; T cells; MYCOBACTERIUM tuberculosis; LYMPHOCYTES
- Publication
Archives of Virology, 2016, Vol 161, Issue 1, p181
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-015-2640-7