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- Title
Integrated physiological, proteome and gene expression analyses provide new insights into nitrogen remobilization in citrus trees.
- Authors
Xiong, Huaye; Ma, Haotian; Zhao, Huanyu; Yang, Linsheng; Hu, Bin; Wang, Jie; Shi, Xiaojun; Zhang, Yueqiang; Rennenberg, Heinz
- Abstract
Nitrogen (N) remobilization is an important physiological process that supports the growth and development of trees. However, in evergreen broad-leaved tree species, such as citrus, the mechanisms of N remobilization are not completely understood. Therefore, we quantified the potential of N remobilization from senescing leaves of spring shoots to mature leaves of autumn shoots of citrus trees under different soil N availabilities and further explored the underlying N metabolism characteristics by physiological, proteome and gene expression analyses. Citrus exposed to low N had an approximately 38% N remobilization efficiency (NRE), whereas citrus exposed to high N had an NRE efficiency of only 4.8%. Integrated physiological, proteomic and gene expression analyses showed that photosynthesis, N and carbohydrate metabolism interact with N remobilization. The improvement of N metabolism and photosynthesis, the accumulation of proline and arginine, and delayed degradation of storage protein in senescing leaves are the result of sufficient N supply and low N remobilization. Proteome further showed that energy generation proteins and glutamate synthase were hub proteins affecting N remobilization. In addition, N requirement of mature leaves is likely met by soil supply at high N nutrition, thereby resulting in low N remobilization. These results provide insight into N remobilization mechanisms of citrus that are of significance for N fertilizer management in orchards.
- Subjects
GENE expression; CITRUS; ORCHARD management; CARBOHYDRATE metabolism; FALL foliage; PROTEOMICS
- Publication
Tree Physiology, 2022, Vol 42, Issue 8, p1628
- ISSN
0829-318X
- Publication type
Article
- DOI
10.1093/treephys/tpac024