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- Title
INHIBITION ACTIVITY OF THUJA OCCIDENTALIS FRUIT EXTRACT AGAINSTS TAPHYLOCOCCUS AUREUS AND E. COLI ISOLATED FROM CASES OF MASTITIS IN BAGHDAD, IRAQ.
- Authors
Al-Roubai, Sahar M. H.; Al-Rawe, Mariam H. A.; Mahmood, Anwar; Khaleefah, Abdulwahhab A.
- Abstract
The study was conducted to investigate a major bacterial cause of mastitis. The research was designed to study the inhibitory effect of Thuja occidentalis against growth of pathogenicbacteriaStaphylococcus aureus has been identified by RapIDTM ANA II System, API Staph Reading Stripe and E. coli by RemelRapIDTM ANA I System compared with standard strain, also diagnosed by biochemical reaction tests. Out of 100 mastitis samples 57 isolates gave positive results, included 31 isolates of S. aureus and 26 of E.coli. The period of research was from February 2019 until September 2019. Current study gave certain significant results about the possibility of Thuja occidentalis fruit extract in its influence against pathogenic bacteria both gram positive (Staphylococcus aureus) and gram negative (E. coli) bacteria). Observations suggested that different concentration (100, 50, 25, 12.5 & 6.25 mg/ml) were of good antibacterial activity against E. coli and S. aureus isolated from cow mastitis, with a significant difference (p<0.05) for some concentrations over others, the peak of effect against gram negative E. coli was at the concentration of 12.5 mg/ml of the aqueous extract and the alcoholic extract as well of Thuja occidentalis fruit, while the minimum effect was at te concentration of 6.25 mg/ml for both aqueous extract and alcoholic extract also. Contrarilly, the gram positive S. aureus bacteria showed the lowest inhibition zone around the extract centre at the same concentration of the extracts (aqueous and alcoholic) when, it was equal to 12.5 mg/ml, at the time that higher effect on the bacterial growth was at the concentration of 6.25 mg/ml. A significant difference was found in the effect ratios between the alcoholic extract and the aqueous extract at the level (p<0.05). As the microbial resistance problem is growing and the global direction went with the use of antimicrobial drugs is still uncertain in the future, therefore, actions must be taken to minimize this problem, by controlling the use of antibiotics and to continue studies for synthesis of new drugs or developing natural ones. Thus, offering efficient and appropriate antimicrobial drugs to human and animal is a global demand.
- Subjects
BAGHDAD (Iraq); FRUIT extracts; THUJA occidentalis; ESCHERICHIA coli; MASTITIS; ANTI-infective agents
- Publication
Biochemical & Cellular Archives, 2020, Vol 20, p3579
- ISSN
0972-5075
- Publication type
Article