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- Title
Centroid of the bacterial growth curves: a metric to assess phage efficiency.
- Authors
Hosseini, Nava; Chehreghani, Mahdi; Moineau, Sylvain; Charette, Steve J.
- Abstract
Phage replication can be studied using various approaches, including measuring the optical density (OD) of a bacterial culture in a liquid medium in the presence of phages. A few quantitative methods are available to measure and compare the efficiency of phages by using a single index based on the analysis of OD curves. However, these methods are not always applicable to non-canonical OD curves. Using the concept of center of area (centroid), we developed a metric called Centroid Index (CI), sensitive to the trend of the growth curves (OD distribution) including bacterial regrowth, which is not considered by the methods already available. We also provide a user-friendly software to facilitate the calculation of CI. This method offers an alternative and more precise way to determine phage efficiency by considering the OD variations over time, which may help in the selection of phages for biocontrol applications. Current methods quantifying phage lytic efficacy have limits. Centroid Index quantifies efficacy via the center of area, offering more accuracy. For calculation of this index, a software called Centroid Index Calculator is presented.
- Subjects
BACTERIAL growth; CENTROID; OPACITY (Optics); BACTERIAL cultures; BACTERIOPHAGES; QUANTITATIVE research
- Publication
Communications Biology, 2024, Vol 7, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-024-06379-z