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- Title
ESTANDARIZACIÓN DEL ANÁLISIS MULTI-LOCUS DE NÚMERO VARIABLE DE REPETICIONES EN TÁNDEM PARA EL ESTUDIO DE Staphylococcus aureus RESISTENTES A METICILINA AISLADOS DE LA COMUNIDAD EN PARAGUAY.
- Authors
Rodríguez-Acosta, Fátima; Fernández, Silvina; Haim, Sol; Mollerach, Marta; Basualdo, Wilma; Castro, Héctor; Quiñónez, Beatriz; María Guillén-Fretes, Rosa
- Abstract
Staphylococcus aureus is a pathogen that can produce several infections with a wide range of severity and it has the ability to adapt to different tissues. The epidemiology is complex, due to circulation of many different clones worldwide, so the analysis for its identification requires reproducible and high discriminatory power molecular methods. The aim of this study was to standardize the molecular technique multiple-locus variable number of tandem repeat analysis (MLVA) for the genetic variability analysis of S. aureus isolates, previously characterized by pulsed field gel electrophoresis (PFGE). The MLVA was made by PCR amplification of seven VNTR locus (clfA, clfB, sdrC, sdrD, sdrE, sspA y spA). A high level of reproducibility has been reached in the study. The use of isolates previously typified by multi-locus sequence typing (MLST), PFGE, locus spa and cassette SCCmec, allowed to validate the MLVA clusters comparatively. The isolates that were clustered by MLVA as the same isolate, showed the same results by other molecular techniques, and the MLVA can distinguish isolates with identical PFGE patterns. This technique meets all the criteria of a useful molecular typification technique.
- Publication
Duazary. Revista de la Facultad de Ciencias de la Salud, 2017, Vol 14, Issue 2, p131
- ISSN
1794-5992
- Publication type
Article
- DOI
10.21676/2389783X.1971