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- Title
Imprinted status of pleomorphic adenoma gene-like I and paternal expression gene 10 genes in pigs.
- Authors
Zhang, F. W.; Cheng, H. C.; Jiang, C. D.; Deng, C. Y.; Xiong, Y. Z.; Li, F. E.; Lei, M. G.
- Abstract
Genomic imprinting is theorized to exist in all placental mammals and some marsupials. Imprinted genes play important roles in the regulation of fetal growth, development, and postnatal behavior, but the study of imprinted genes has been limited in livestock. In this study, the polymorphism-based approach was used to detect the expression patterns of the porcine pleomorphic adenoma gene-like I (PLAGL1) and paternal expression gene 10 (PEG10) genes. Single nucleotide polymorphisms in the exons were detected between the Meishan and Large White breeds in the PLAGL1 and PEG10 genes. The polymorphisms were used to determine the monoallelic or biallelic expression with reverse transcription-PCR-RFLP in 44 tissues from 4 heterozygous pigs (based on SNP). Imprinting analysis indicated that the PLAGL1 and PEG10 genes were both paternally expressed in all tissues tested (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, and ovary). Our study showed that the method of identifying polymorphic transcripts with reverse transcription-PCR-RFLP may be beneficial for detecting the imprinting status of some candidate imprinted genes.
- Subjects
GENES; GENOMIC imprinting; SWINE; GENE expression; GENETIC polymorphisms; MARSUPIALS
- Publication
Journal of Animal Science, 2007, Vol 85, Issue 4, p886
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2006-278