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- Title
Production of a polyclonal antibody against unprocessed chicken myostatin and the effects of in-ovo administration of the antibody on post-hatch broiler growth and muscle mass.
- Authors
Bobbili, N. K.; Lee, Y. K.; Kim, Y. S.
- Abstract
Myostatin, a member of the TGF-β superfamily, is a potent negative regulator of skeletal muscle growth. The objective of this study was to produce a polyclonal antibody against unprocessed chicken myostatin and to examine the effects of in-ovo administration of the antibody on post-hatch broiler growth and muscle mass. Unprocessed form of chicken myostatin, which had been expressed in E. coli and purified by electro-elution of myostatin bands after fractionation by SDS-PAGE, was used as an immunogen in producing a polyclonal antibody against unprocessed myostatin in rabbit. In Western blot analysis, the antibody showed a strong binding affinity to commercially available myostatin prodomain with little binding affinity to mature myostatin. The antibody also demonstrated a certain level of cross-reactivity with pTGF-β1 and rhBMP2, but not with pTGF-β2, rhTGF-β3, rhBMP3 and rhBMP5 in Western blot analysis. To examine the effects of in-ovo administration of the antibody, eggs were injected once with 35 μg antibody in 50 μL PBS per egg either into the albumen (Alb) or yolk (Yolk) on day 3 of incubation. Controls (Con) received no injection. After hatch, chicks were raised for 28 d. The broilers of the Yolk group had significantly (P<0.05) lower body wt (8.5%) at 7 d than the Con group. At 14, 21 and 28 d, the mean body wt of the Yolk group was lower (5%) than that of the Con, but the difference was not statistically significant. Thigh and leg muscle wt of the Yolk group was significantly (P<0.05) lower (10%) than that of the Con at 28 d. In contrast, no significant effects on body and muscle mass were observed when the antibody was injected into the albumen. In summary, the polyclonal antibody raised against the unprocessed chicken myostatin binds to myostatin prodomain, and injection of this antibody to the yolk of eggs appeared to decrease muscle mass in chicks hatched from these eggs.
- Subjects
PUBLIC Broadcasting Service (U.S.); MUSCLE mass; MYOSTATIN; MUSCLE growth; ANTIBODY formation; EGG incubation; LEG muscles; WESTERN immunoblotting; EGG yolk
- Publication
Journal of Animal Science, 2006, Vol 84, p30
- ISSN
0021-8812
- Publication type
Article