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- Title
PKA-activated ApAF-ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation.
- Authors
Jin-A Lee; Sue-Hyun Lee; Changhoon Lee; Deok-Jin Chang; Yong Lee; Hyoung Kim; Ye-Hwang Cheang; Hyoung-Gon Ko; Yong-Seok Lee; Heejung Jun; Bartsch, Dusan; Kandel, Eric R.; Bong-Kiun Kaang
- Abstract
Long-term memory requires transcriptional regulation by a combination of positive and negative transcription factors. Aplysia activating factor (ApAF) is known to be a positive transcription factor that forms heterodimers with ApC/EBP and ApCREB2. How these heterodimers are regulated and how they participate in the consolidation of long-term facilitation (LTF) has not, however, been characterized. We found that the functional activation of ApAF required phosphorylation of ApAF by PKA on Ser-266. In addition, ApAF lowered the threshold of LTF by forming a heterodimer with ApCREB2. Moreover, once activated by PKA, the ApAF-ApC/EBP heterodimer transactivates enhancer response element--containing genes and can induce LTF in the absence of CRE- and CREB-mediated gene expression. Collectively, these results suggest that PKA-activated ApAF-ApC/EBP heterodimer is a core downstream effector of ApCREB in the consolidation of LTF.
- Subjects
LONG-term memory; MEMORY; TRANSCRIPTION factors; PHOSPHORYLATION; CHEMICAL reactions
- Publication
Journal of Cell Biology, 2006, Vol 174, Issue 6, p827
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200512066