We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Protein synthesis persists during necrotic cell death.
- Authors
Saelens, Xavier; Festjens, Nele; Parthoens, Eef; Vanoverberghe, Isabel; Kalai, Michael; Van Kuppeveld, Frank; Vandenabeele, Peter
- Abstract
Cell death is an intrinsic part of metazoan development and mammalian immune regulation. Whereas the molecular events orchestrating apoptosis have been characterized extensively, little is known about the biochemistry of necrotic cell death. Here, we show that, in contrast to apoptosis, the induction of necrosis does not lead to the shut down of protein synthesis. The rapid drop in protein synthesis observed in apoptosis correlates with caspase-dependent breakdown of eukaryotic translation initiation factor (elF) 4G, activation of the double-stranded RNA-activated protein kinase PKR, and phosphorylation of its substrate eIF2-α. In necrosis induced by tumor necrosis factor, double-stranded RNA, or viral infection, de novo protein synthesis persists and 28S ribosomal RNA fragmentation, eIF2-α phosphorylation, and proteolytic activation of PKR are absent. Collectively, these results show that, in contrast to apoptotic cells, necrotic dying cells retain the opportunity to synthesize proteins.
- Subjects
PROTEIN synthesis; CELL death; NECROSIS; IMMUNOREGULATION; METAZOA; MAMMALS
- Publication
Journal of Cell Biology, 2005, Vol 168, Issue 4, p545
- ISSN
0021-9525
- Publication type
Abstract
- DOI
10.1083/jcb.200407162