We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2.
- Authors
Boix-Besora, Arnau; Lorenzo, Elianet; Lavado-García, Jesús; Gòdia, Francesc; Cervera, Laura
- Abstract
Virus-like particles (VLPs) constitute a promising approach to recombinant vaccine development. They are robust, safe, versatile and highly immunogenic supra-molecular structures that closely mimic the native conformation of viruses without carrying their genetic material. HIV-1 Gag VLPs share similar characteristics with wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, making them a suitable platform for the expression of its spike membrane protein to generate a potential vaccine candidate for COVID-19. This work proposes a methodology for the generation of SARS-CoV-2 VLPs by their co-expression with HIV-1 Gag protein. We achieved VLP functionalization with coronavirus spike protein, optimized its expression using a design of experiments (DoE). We also performed the bioprocess at a bioreactor scale followed by a scalable downstream purification process consisting of two clarifications, an ion exchange and size-exclusion chromatography. The whole production process is conceived to enhance its transferability at current good manufacturing practice (cGMP) industrial scale manufacturing. Moreover, the approach proposed could be expanded to produce additional Gag-based VLPs against different diseases or COVID-19 variants.
- Subjects
SARS-CoV-2; VIRUS-like particles; CURRENT good manufacturing practices; HIV; ION exchange chromatography
- Publication
Vaccines, 2022, Vol 10, Issue 2, p250
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines10020250