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- Title
Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.
- Authors
Soreanu, Inga; Hendler, Adi; Dahan, Danielle; Dovrat, Daniel; Aharoni, Amir
- Abstract
The budding yeast is currently one of the major model organisms for the study of a wide variety of biological processes. Genetic manipulation of yeast involves the extensive usage of selectable markers that can lead to undesired effects. Thus, marker-free genetic manipulation in yeast is highly desirable for gene/promoter replacement and various other applications. Here we combine the power of selectable markers followed by CRISPR/CAS9 genome editing for common genetic manipulations in yeast in a marker-free manner. We demonstrate our approach for whole gene and promoter replacements and for high-efficiency operator array integration. Our approach allows the utilization of many thousands of existing strains including library strains for the generation of significant genetic changes in yeast in a marker-free and cloning-free fashion.
- Subjects
CRISPRS; GENOME editing; GENETIC markers; SACCHAROMYCES cerevisiae; YEAST genetic engineering
- Publication
Current Genetics, 2018, Vol 64, Issue 5, p1129
- ISSN
0172-8083
- Publication type
Article
- DOI
10.1007/s00294-018-0831-y