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- Title
Role of protein kinase C delta in X-ray-induced apoptosis of keratinocyte.
- Authors
Young-Sook Lee; Kyung-Cheol Sohn; Ki-Hwan Kim; Moon-June Cho; Gang Min Hur; Tae-Jin Yoon; Sung Kyu Kim; Kyungmoon Lee; Jeung-Hoon Lee; Chang Deok Kim
- Abstract
In this study, we investigated the process of X-ray-induced apoptosis of skin keratinocyte, and the functional role of protein kinase C delta (PKCδ) and downstream signalling cascade. High-dose X-ray irradiation (10 Gy) led to the apoptosis of HaCaT keratinocyte, accompanied by PKCδ cleavage. Treatment with PKCδ inhibitor and adenoviral transduction of dominant-negative PKCδ clearly inhibited the X-ray-induced apoptosis of keratinocyte. In addition, X-ray induced the phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2) and inhibition by ERK1/2 inhibitor abrogated the X-ray-induced apoptosis. Interestingly, overexpression of dominant-negative PKCδ markedly blocked the X-ray-induced phosphorylation of ERK1/2, suggesting that ERK1/2 is the functional downstream effector of PKCδ. Next, we investigated the difference between UVB and X-ray response. UVB induced the apoptosis of keratinocyte in a PKCδ-dependent manner, similar to X-ray response. However, UVB irradiation induced the phosphorylation of c-jun N-terminal kinases (JNK) and inhibition of JNK significantly protected the UVB-induced apoptosis. These results demonstrate that PKCδ is a key regulator in X-ray-induced apoptosis of keratinocyte and suggest that there is subtle difference in downstream signalling cascade between UVB and X-ray response of keratinocyte.
- Subjects
PROTEIN kinases; APOPTOSIS; KERATINOCYTES; PHOSPHORYLATION; IRRADIATION
- Publication
Experimental Dermatology, 2009, Vol 18, Issue 1, p50
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/j.1600-0625.2008.00761.x