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- Title
ETHYLENE RESPONSE FACTOR 74 ( ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis.
- Authors
Yao, Yuan; He, Run Jun; Xie, Qiao Li; Zhao, Xian hai; Deng, Xiao mei; He, Jun bo; Song, Lili; He, Jun; Marchant, Alan; Chen, Xiao‐Yang; Wu, Ai‐Min
- Abstract
Recent studies indicate that the ETHYLENE RESPONSE FACTOR VII (ERF-VII) transcription factor is an important regulator of osmotic and hypoxic stress responses in plants. However, the molecular mechanism of ERF-VII-mediated transcriptional regulation remains unclear., Here, we investigated the role of ERF74 (a member of the ERF-VII protein family) by examining the abiotic stress tolerance of an ERF74 overexpression line and a T-DNA insertion mutant using flow cytometry, transactivation and electrophoretic mobility shift assays., 35S:: ERF74 showed enhanced tolerance to drought, high light, heat and aluminum stresses, whereas the T-DNA insertion mutant erf74 and the erf74;erf75 double mutant displayed higher sensitivity. Using flow cytometry analysis, we found that erf74 and erf74;erf75 lines lack the reactive oxygen species (ROS) burst in the early stages of various stresses, as a result of the lower expression level of RESPIRATORY BURST OXIDASE HOMOLOG D ( RbohD). Furthermore, ERF74 directly binds to the promoter of RbohD and activates its expression under different abiotic stresses. Moreover, induction of stress marker genes and ROS-scavenging enzyme genes under various stress conditions is dependent on the ERF74− RbohD−ROS signal pathway., We propose a pathway that involves ERF74 acting as an on-off switch controlling an RbohD-dependent mechanism in response to different stresses, subsequently maintaining hydrogen peroxide (H2O2) homeostasis in Arabidopsis.
- Subjects
ABIOTIC stress; ABIOTIC environment; ARABIDOPSIS thaliana; REACTIVE oxygen species; FREE radicals
- Publication
New Phytologist, 2017, Vol 213, Issue 4, p1667
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14278