We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A membrane antibody receptor for noninvasive imaging of gene expression.
- Authors
Roffler, S. R.; H.-E. Wang; H.-M. Yu; W.-D. Chang; C.-M. Cheng; Y.-L. Lu; B.-M. Chen; T.-L. Cheng
- Abstract
Monitoring gene expression is important to optimize gene therapy protocols and ensure that the proper tissue distribution is achieved in clinical practice. We developed a noninvasive imaging system based on the expression of artificial antibody receptors to trap hapten-labeled imaging probes. Functional membrane-bound anti-dansyl antibodies (DNS receptor) were stably expressed on melanoma cells in vitro and in vivo. A bivalent (DNS)2-diethylenetriaminepentaacetic 111Indium probe specifically bound to cells that expressed DNS receptors but not control scFv receptors. Importantly, the 111In probe preferentially localized to DNS receptors but not control receptors on tumors in mice as assessed by gamma camera imaging. By 48 h after intravenous injection, the uptake of the probe in tumors expressing DNS receptors was 72 times greater than the amount of probe in the blood. This targeting strategy may allow noninvasive assessment of the location, extent and persistence of gene expression in living animals and in the clinic.Gene Therapy (2006) 13, 412–420. doi:10.1038/sj.gt.3302671; published online 3 November 2005
- Subjects
GENE expression; GENE therapy; DIAGNOSTIC imaging; MELANOMA; CLINICAL medicine; TUMORS
- Publication
Gene Therapy, 2006, Vol 13, Issue 5, p412
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3302671