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- Title
Expression of 15-PGDH is downregulated by COX-2 in gastric cancer.
- Authors
Zhenxiong Liu; Xin Wang; Yuanyuan Lu; Shuang Han; Faming Zhang; Huihong Zhai; Ting Lei; Jie Liang; Jun Wang; Kaichun Wu; Daiming Fan
- Abstract
To explore the proteins regulated by cyclooxygenase-2 (COX-2) in gastric cancer, the expression plasmid of COX-2siRNA was constructed and transfected into gastric cancer cell line SGC7901. Then, two-dimensional electrophoresis and the PDQuest software analysis were applied to discover the differentially expressed proteins. The differential protein spots were analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Fourteen differentially expressed proteins between the two cell lines were identified. 15-Hydroxyprostaglandin dehydrogenase [NAD+] (15-PGDH), a key enzyme in prostaglandin degradation, was identified as an upregulated protein in SGC7901 cells transfected with the COX-2siRNA plasmid. To further explore whether the 15-PGDH is regulated by COX-2, western blotting and immunocytochemical assay were performed to detect the expression of 15-PGDH in different cell lines with different expression level of COX-2. The results showed that the expression of 15-PGDH was upregulated (128.57%) as COX-2 was suppressed by small interfering RNA and downregulated (51.72%) as COX-2 was enhanced by COX-2 cDNA transfection in gastric cancer cells. In tissue specimens with gastric cancer, there was a decreased expression of 15-PGDH and an increased expression of COX-2 simultaneously. A significantly negative correlation of 15-PGDH expression was found to COX-2 level, tumor differentiation, tumor, lymph node, metastasis (TNM) staging and lymph node metastasis of gastric cancer. All the results suggest that 15-PGDH is downregulated by COX-2 in human gastric cancer and may contribute to the carcinogenesis and development of human gastric cancer in combination with COX-2.
- Subjects
GENETIC regulation; GENE expression; CYCLOOXYGENASE 2; STOMACH cancer; SMALL interfering RNA; TIME-of-flight mass spectrometry; TWO-dimensional electrophoresis; GENE transfection; GENETICS
- Publication
Carcinogenesis, 2008, Vol 29, Issue 6, p1219
- ISSN
0143-3334
- Publication type
Article
- DOI
10.1093/carcin/bgm297