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- Title
Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases.
- Authors
Kawada, Taishu; Kino, Katsuhito; Tokorodani, Kyousuke; Anabuki, Ryuto; Morikawa, Masayuki; Kobayashi, Takanobu; Ohara, Kazuaki; Ohshima, Takayuki; Miyazawa, Hiroshi
- Abstract
Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua. We report here the following nucleotide incorporations opposite Ua by various DNA polymerases: DNA polymerase α, dATP and dGTP (dATP > dGTP); DNA polymerase δ, dATP; DNA polymerase ζ, dATP; Kf exo−, dATP; Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), dGTP and dATP (dGTP > dATP); and DNA polymerase η, dCTP, dGTP, dATP, and dTTP (dCTP > dGTP > dATP > dTTP). DNA polymerases β and ε were blocked by Ua. Elongation by DNA polymerases δ and ζ stopped after inserting dATP opposite Ua. Importantly, the elongation efficiency to full-length beyond Ua using DNA polymerase η and Dpo4 were almost the same as that of natural DNA.
- Subjects
UREA; DNA polymerases; THYMINE; GUANINE; BASE pairs
- Publication
Genes & Environment, 2022, Vol 44, Issue 1, p1
- ISSN
1880-7046
- Publication type
Article
- DOI
10.1186/s41021-022-00236-3