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- Title
Effect of the acute heat stress on serum endotoxin concentration and the expression of TLR4 mRNA in liver of Arbor Acres broiler chickens.
- Authors
Huang, Shu-cheng; Fu, Ya-Fen; Rehman, Mujeeb Ur; Li, Kun; Lan, Yan-fang; Liu, Wei; Luo, Hou-qiang; Zhang, Hui; Zhang, Li-hong; Hao, Ya-nan; Tong, Zong-xi
- Abstract
This study was undertaken to investigate the relationship between gut-derived endotoxin and the Toll-like receptor 4 (TLR4) expression in the liver of broilers under acute heat stress (AHS). For this purpose, 120 Arbor Acres chicks were randomised into two groups: control temperature group (CT group, 22 ± 1°C) and high temperature group (HT group, 38 ± 1°C). The chicks received AHS at Day 28 and their small intestine, liver and blood samples were collected after 2 h, 5 h and 10 h to examine the histopathology, biochemical parameters, endotoxin concentrations and TLR4 expression. The results showed that damaged intestinal villi and severe congestion of the hepatic sinusoids were observed, especially after 10 h of AHS in the HT group. In addition, the levels of alanine transferase, aspartate transaminase, and direct bilirubin, except alkaline phosphataseafter were significantly elevated (P < 0.05) and total bilirubin (P < 0.01) and albumin (P < 0.05) were decreased after 10 h of AHS as compared with the CT group, which are associated with liver function. Moreover, the mRNA expression of TLR4 in the liver was noticeably upregulated (P < 0.05) during AHS with significantly increased in endotoxin concentration (P < 0.01) of broilers. Altogether, these findings suggest that the upregulated expression of TLR4 mRNA was triggered via gut-derived endotoxin in heat stress-induced liver injury. Heat stress is a very challenging environmental condition for broiler chickens that has caused considerable economic losses worldwide to the poultry industry. To investigate the relationship between gut-derived endotoxin and liver injury via detecting Toll-like receptor 4 (TLR4) expression, the heat stress experiment of broiler chickens found that the upregulated TLR4 expression was triggered via gut-derived endotoxin in heat stress-induced liver injury. This may provide new insights for the therapeutic manipulation of heat stress in poultry.
- Subjects
PHYSIOLOGICAL effects of heat; ENDOTOXINS; BROILER chickens
- Publication
Animal Production Science, 2018, Vol 58, Issue 12, p2200
- ISSN
1836-0939
- Publication type
Article
- DOI
10.1071/AN16423