We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane.
- Authors
Lv, Huixin; Xu, Jing; Wang, Yihan; Liu, Xiuyu; Chen, Sheng; Chen, Jingxia; Zhai, Jingjie; Zhou, Yanmin
- Abstract
Aim: To discover the populations of mesenchymal stem cells (MSCs) derived from different layers of human maxillary sinus membrane (hMSM) and evaluate their osteogenic capability. Materials and Methods: hMSM was isolated into a monolayer using the combined method of physical separation and enzymatic digestion. The localization of MSCs in hMSM was performed by immunohistological staining and other techniques. Lamina propria layer‐derived MSCs (LMSCs) and periosteum layer‐derived MSCs (PMSCs) from hMSM were expanded using the explant cell culture method and identified by multilineage differentiation assays, colony formation assay, flow cytometry and so on. The biological characteristics of LMSCs and PMSCs were compared using RNA sequencing, reverse transcription and quantitative polymerase chain reaction, immunofluorescence staining, transwell assay, western blotting and so forth. Results: LMSCs and PMSCs from hMSMs were both CD73‐, CD90‐ and CD105‐positive, and CD34‐, CD45‐ and HLA‐DR‐negative. LMSCs and PMSCs were identified as CD171+/CD90+ and CD171−/CD90+, respectively. LMSCs displayed stronger proliferation capability than PMSCs, and PMSCs presented stronger osteogenic differentiation capability than LMSCs. Moreover, PMSCs could recruit and promote osteogenic differentiation of LMSCs. Conclusions: This study identified and isolated two different types of MSCs from hMSMs. Both MSCs served as good potential candidates for bone regeneration.
- Subjects
CYTOLOGY; IN vitro studies; FLOW cytometry; BONE regeneration; RESEARCH funding; CELL membranes; MESENCHYMAL stem cells; CELL proliferation; BONE growth; POLYMERASE chain reaction; FLUORESCENT antibody technique; DESCRIPTIVE statistics; MANN Whitney U Test; MAXILLARY sinus; IMMUNOHISTOCHEMISTRY; CELL culture; MESSENGER RNA; HISTOLOGICAL techniques; WESTERN immunoblotting; ONE-way analysis of variance; CELL differentiation; STAINS &; staining (Microscopy); DATA analysis software; CELL separation; SEQUENCE analysis
- Publication
Journal of Clinical Periodontology, 2024, Vol 51, Issue 6, p754
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/jcpe.13956