We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Oral bacteriome of HIV‐1‐infected children from Rio de Janeiro, Brazil: Next‐generation DNA sequencing analysis.
- Authors
Gonçalves, Lucio Souza; Ferreira, Dennis de Carvalho; Heng, Nicholas C. K.; Vidal, Fabio; Santos, Henrique Fragoso; Zanicotti, Diogo Godoy; Vasconcellos, Mariana; Stambovsky, Mayra; Lawley, Blair; Rubini, Norma de Paula Motta; Santos, Katia Regina Netto; Seymour, Gregory J.
- Abstract
Background and Aim: This study compared the oral bacteriome between HIV‐1‐infected and non‐HIV‐1‐infected Brazilian children/teenagers. Methods: Whole saliva, biofilm from the dorsal surface of the tongue and biofilm from supragingival and subgingival sites were collected from 27 HIV‐1‐infected and 30 non‐HIV‐1‐infected individuals. Bacterial genomic DNA was extracted and 16S rRNA genes were sequenced using next‐generation sequencing technology (Ion Torrent). Results: In the supragingival biofilm, the phylum Firmicutes and genus Streptococcus sp. were more frequent in HIV‐1‐infected (95% and 78%, respectively) than in non‐HIV‐1‐infected individuals (40% and 24%, respectively). In the subgingival biofilm of HIV‐infected participants, the relative abundance of the Veillonella sp. and Prevotella sp. genera were higher than in non‐HIV‐1‐infected participants. On the tongue, the genera with greater relative abundance in HIV‐1‐infected individuals were Neisseria sp. (21%). In saliva, the difference of the genus Prevotella sp. between non‐HIV‐1‐infected and HIV‐1‐infected individuals was 15% and 7%, respectively. The Chao index revealed an increase in the richness of both sub‐ and supragingival biofilms in the HIV‐1‐infected samples compared with non‐HIV‐1‐infected samples. Conclusion: HIV‐1‐infected children/teenagers have a higher frequency of the phyla Firmicutes and genus Streptococcus, and their oral microbiome shows more complexity than that of non‐HIV‐1‐infected children/teenagers.
- Subjects
BRAZIL; NUCLEOTIDE sequencing; HIV-positive children; ORAL microbiology; DNA analysis; SALIVA analysis; PERIODONTAL disease; STREPTOCOCCUS
- Publication
Journal of Clinical Periodontology, 2019, Vol 46, Issue 12, p1192
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/jcpe.13176