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- Title
DNA methylation changes at SNCA intron 1 in patients with dementia with Lewy bodies.
- Authors
Funahashi, Yu; Yoshino, Yuta; Yamazaki, Kiyohiro; Mori, Yoko; Mori, Takaaki; Ozaki, Yuki; Sao, Tomoko; Ochi, Shinichiro; Iga, Jun‐ichi; Ueno, Shu‐ichi
- Abstract
Aim It is difficult to diagnose dementia with Lewy bodies ( DLB) because it exhibits clinical and neuropathological overlap with both Alzheimer's disease and Parkinson's disease. The α-synuclein protein is a major component of Lewy bodies, and accumulation of α-synuclein aggregates causes synaptic dysfunction in DLB. Epigenetic changes at the synuclein alpha ( SNCA) gene may be involved in DLB pathogenesis. Methods We examined DNA methylation rates at 10 CpG sites located in intron 1 of SNCA and SNCA m RNA expression in peripheral leukocytes to compare DLB patients ( n = 20; nine men, 11 women; age = 78.8 ± 7.7 years) with healthy controls ( n = 20; eight men, 12 women; age = 77.0 ± 6.9 years). Results The methylation rate at CpG 4 ( P = 0.002) and the overall mean methylation rate at these sites ( P < 0.001) were significantly lower in DLB patients than in healthy controls after Bonferroni correction. Although SNCA126, a partial form of SNCA m RNA expression, was significantly increased in DLB ( P = 0.017), there was no significant difference in total SNCA m RNA expression between DLB patients and healthy controls ( P = 0.165). No correlation was observed between SCNA m RNA expression levels and blood DNA methylation rates in either DLB or healthy controls. Conclusion Our findings indicated that lower methylation rates may be a biomarker for DLB.
- Subjects
DNA methylation; LEWY body dementia; INTRONS; DEMENTIA; PARKINSONIAN disorders
- Publication
Psychiatry & Clinical Neurosciences, 2017, Vol 71, Issue 1, p28
- ISSN
1323-1316
- Publication type
Article
- DOI
10.1111/pcn.12462