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- Title
Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis.
- Authors
Hu, Zhi-qiang; Yao, Yong-ming; Chen, Wei; Bian, Jia-lan; Zhao, Lin-jun; Chen, Long-wang; Hong, Guang-liang; Lu, Zhong-qiu; Zhao, Guang-ju
- Abstract
Immune dysfunction contributes to secondary infection and worse outcomes in sepsis. Regulatory T cells (Tregs) have been implicated in sepsis-induced immunosuppression. Nevertheless, the role of Tregs in secondary infection after sepsis remains to be determined. In the present study, a two-hit model which mimics clinical conditions was used and the potential role of Tregs in secondary Pseudomonas aeruginosa infection post-sepsis was investigated. Results showed that mice were susceptible to secondary P. aeruginosa infection 3 days, but not 7 days, post-cecal ligation and puncture (CLP). The levels of IL-17A, IL-1β, and IL-6 remained low in CLP mice after P. aeruginosa infection, while the levels of IL-10 increased significantly. Additionally, increased number of Tregs in both lung and spleen was observed in “two-hit” mice. Injection with PC61 (anti-CD25) mAb reduced the number of Tregs by 50% in spleen and 60% in lung of septic mice. This partial depletion of Tregs elevated IL-17A, IL-1β, and IL-6 production and decreased IL-10 levels in septic mice with P. aeruginosa infection, leading to lower bacterial load, attenuation of lung injury, and improvement of survival. The present findings demonstrate that Tregs play a crucial role in secondary P. aeruginosa infection after sepsis by modulating the inflammatory response.
- Subjects
PSEUDOMONAS aeruginosa infections; SEPSIS; T cells; INFLAMMATION; INTERLEUKINS
- Publication
Inflammation, 2018, Vol 41, Issue 5, p1780
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-018-0821-8