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- Title
Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects.
- Authors
Tran, My Hanh; Nguyen, Thi Van Anh; Do, Hoang Giang; Kieu, Trung Kien; Nguyen, Thi Kim Thanh; Le, Hong Diep; Guerrero-Limon, Gustavo; Massoz, Laura; Nivelle, Renaud; Zappia, Jérémie; Pham, Hai The; Nguyen, Lai Thanh; Muller, Marc
- Abstract
Evaluating the risks and benefits of using traditional medicinal plants is of utmost importance for a huge fraction of the human population, in particular in Northern Vietnam. Zebrafish are increasingly used as a simple vertebrate model for testing toxic and physiological effects of compounds, especially on development. Here, we tested 12 ethanolic extracts from popular medicinal plants collected in northern Vietnam for their effects on zebrafish survival and development during the first 4 days after fertilization. We characterized more in detail their effects on epiboly, hatching, growth, necrosis, body curvature, angiogenesis, skeletal development and mostly increased movement behavior. Finally, we confirm the effect on epiboly caused by the Mahonia bealei extract by staining the actin filaments and performing whole genome gene expression analysis. Further, we show that this extract also inhibits cell migration of mouse embryo fibroblasts. Finally, we analyzed the chemical composition of the Mahonia bealei extract and test the effects of its major components. In conclusion, we show that traditional medicinal plant extracts are able to affect zebrafish early life stage development to various degrees. In addition, we show that an extract causing delay in epiboly also inhibits mammalian cell migration, suggesting that this effect may serve as a preliminary test for identifying extracts that inhibit cancer metastasis.
- Subjects
VIETNAM; BRACHYDANIO; ZEBRA danio embryos; MEDICINAL plants; EMBRYOS; PLANT extracts; CELL migration; PLANT collecting
- Publication
PLoS ONE, 2023, Vol 18, Issue 11, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0294048