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- Title
PROBING BIOCIDE PENETRATION AND RETENTION IN WOOD PRODUCTS BY IMMUNOLABELING TECHNIQUES.
- Authors
RUEL, Katia; TAPIN-LINGUA, Sandra; MESSAOUDI, Daouïa; FAHY, Olivier; JEQUEL, Marc; JOSELEAU, Jean-Paul; PETIT-CONIL, Michel
- Abstract
Wood products are commonly degraded by decay fungi, insects (including termites), or other organisms (marine borers). Chemical treatments with biocides are the most effective way to protect wood from these organisms. Surface treatments by immersion, aspersion, spraying, brushing, dipping and injection are extensively used to apply the biocidal products to the wood. The performance of preservative treatments is often evaluated according to their retention and penetration. It is therefore important, for either quality control or research and development purposes, to study the penetration capacity of a biocidal product. The methods commonly used for determining penetration of wood preservatives include observing their migration through their own color, or through color indicators, or extracting samples followed by chemical detection by chromatography analysis. However, these multistep methods are time consuming and do not allow the detection and precise location of active substances within the wood structure. Here, we present the general principle of a novel specific approach for detection, visualization and ultrastructural localization of biocidal active substances in treated wood using immunological techniques together with electron microscopy. In the present work, we raised a polyclonal antibody against cypermethrin and applied it for the detection of the pyrethroid insecticide in Pinus sylvestris samples and its localization within the wood cell walls by transmission electron microscopy. Thus, the distance of penetration of cypermethrin from the sample surface could be evaluated and the results were correlated with chemical analysis. The interest of the immunological detection method is discussed.
- Subjects
WOOD-decaying fungi; BIOCIDES; CHROMATOGRAPHIC analysis; MARINE borers; IMMERSIONS (Mathematics)
- Publication
Pro Ligno, 2015, Vol 11, Issue 4, p216
- ISSN
1841-4737
- Publication type
Article