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- Title
Endogenous calmodulin interacts with the epidermal growth factor receptor in living cells
- Authors
Li, Hongbing; Ruano, María José; Villalobo, Antonio
- Abstract
We have previously shown that exogenous calmodulin (CaM) binds to the epidermal growth factor receptor (EGFR) at its cytosolic juxtamembrane region inhibiting its tyrosine kinase activity. We demonstrate in this report that endogenous CaM binds to EGFR in intact cells as CaM co-immunoprecipitates with EGF-activated and non-activated receptors. We also show in living cells that cell-permeable CaM inhibitors prevent the full transphosphorylation of wild type EGFR but not the transphosphorylation of an insertional EGFR mutant in which the CaM-binding domain was divided into two parts. Overall these results suggest that CaM interacts with EGFR in vivo.
- Subjects
CALMODULIN; EPIDERMAL growth factor; PROTEIN-tyrosine kinases; CELL receptors
- Publication
FEBS Letters, 2004, Vol 559, Issue 1-3, p175
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00067-5