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- Title
Do H<sup>+</sup> ions obscure electrogenic Na<sup>+</sup> and K<sup>+</sup> binding in the E<sub>1</sub> state of the Na,K-ATPase?
- Authors
Apell, Hans-Jürgen; Diller, Anna
- Abstract
In contrast to other P-type ATPases, the Na,K-ATPase binding and release of ions on the cytoplasmic side, to the state called E1, is not electrogenic with the exception of the third Na+. Since the high-resolution structure of the closely related SR Ca-ATPase in state E1 revealed the ion-binding sites deep inside the transmembrane part of the protein, the missing electrogenicity in state E1 can be explained by an obscuring counter-movement of H+ ions. Evidence for such a mechanism is presented by analysis of pH effects on Na+ and K+ binding and by electrogenic H+ movements in the E1 conformation of the Na,K-ATPase.
- Subjects
BINDING sites; ADENOSINE triphosphatase
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p198
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03675-X