We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
PP1/PP2A phosphatases inhibitors okadaic acid and calyculin A block ERK5 activation by growth factors and oxidative stress
- Authors
Garcia, Lourdes; Garcia, Fatima; Llorens, Franc; Unzeta, Mercedes; Itarte, Emilio; Gómez, Néstor
- Abstract
Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observed in HeLa cells exposed to epidermal growth factor and H2O2 as well as in PC12 cells stimulated by nerve growth factor and H2O2. Calyculin A, another PP1 and PP2A inhibitor, behaved similarly although these compounds are not structurally related. This suggests that either PP1 or PP2A or both are necessary for ERK5 activation. Protein kinase C (PKC) acts as a negative regulator of the ERK5 activation pathway, however our data suggest that the effects of PKC and the phosphatase are unrelated.
- Subjects
PHOSPHOPROTEIN phosphatases; PROTEIN kinases; GROWTH factors; HELA cells
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p90
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02950-2