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- Title
Effect of Antibacterial Factors and Biostimulators of Cell Metabolism on Bacterial Luminescence.
- Authors
Pshenichnov, R. A.; Maslennikova, I. L.; Nikitina, N. M.
- Abstract
Bioluminescence was used as an index of effects of a series of factors (differing in origin and structure) on cell metabolism. Luminescence of intact cells of Escherichia coli lum+ (a genetically modified strain) was measured on exposure to antibiotics, probiotics, phages, and biostimulators. The sensitivity of E. coli lum+ to antibiotics correlated with antibiotic-induced luminescence quenching. Bioluminescence-based assessment of the antagonistic activity of the main probiotics (bificol, bifidobacterin, acylact, colibacterin, and a composite probiotic preparation), each taken at a concentration of 1 dose/ml, demonstrated that bacterial luminescence was inhibited by 75–99.9% (exposure time, 30 min). Bioluminescence changes reflected cell damage associated with phage infection. It was shown that bioluminescence stimulation could be used as an index of the effect of immunomodulators (olexin and vermin) on cell metabolism in bacterial cultures.
- Subjects
ESCHERICHIA coli; LUMINOUS bacteria; BIOLUMINESCENCE; CELL metabolism; METABOLISM; MICROBIOLOGY; BIOCHEMISTRY
- Publication
Applied Biochemistry & Microbiology, 2003, Vol 39, Issue 3, p270
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1023/A:1023575527240