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- Title
DETECTION OF COMPLEX VERTEBRAL MALFORMATION CARRIERS IN SLOVAK HOLSTEIN CATTLE BY HIGH RESOLUTION MELTING ANALYSIS.
- Authors
Gábor, M.; Martina, Miluchová; Anna, Trakovická; Zuzana, Riecká; Candrák, J.; Klára, Vavrisínová
- Abstract
The objective of this study was to apply high resolution melting analysis in the detection of complex vertebral malformation (CVM) carriers in Hosltein cattle. A total of 47 animals of Holstein cattle were included in this study Genomic DNA was extracted from blood, hair follicles and sperm by commercial QIAamp® DNA Mini kit. The amplification and high resolution melting analysis (HRMA) was done by commercial SensiMix™ HRM kit. The confirmation of sensitivity of this method was done by PCR-PIRA method and sequencing. Four samples of heterozygous genotype GT for causal mutation in the bovine solute carrier family 35 member 3 gene (SLC35A3), which is responsible for CVM disease, were detected. Our results demostrated that the use of HRMA for genotyping of mutant allele T for SLC35A3 gene in Holstein cattle is an effective method for the selection of carriers of CVM disease.
- Subjects
VERTEBRAE abnormalities; HOLSTEIN-Friesian cattle; DNA; ALLELES; SPERMATOZOA; ABNORMALITIES in animals; CATTLE
- Publication
Acta Veterinaria, 2012, Vol 62, Issue 2/3, p239
- ISSN
0567-8315
- Publication type
Article
- DOI
10.2298/AVB1203239G