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- Title
Molecular Modification of AgNPs Synthetic Protein and Characterization of Synthetic AgNPs.
- Authors
Chai, Tong; Song, Yang Ping; Zhang, Sheng Tao; Yan, Fu Wei; Pan, Gong Ying; Liu, Pei; Wang, Shi Yan; li, Xiang Qian
- Abstract
Nanosilver (AgNPs) exhibit a wide range of potential uses in the optical, antibacterial, and catalytic fields, and biologically produced AgNPs have a number of benefits. Our lab earlier identified the Lysinibacillus sphaericus protein NA33, which is involved in the synthesis of AgNPs. Nevertheless, NA33 produced AgNPs with poor dispersion, a high particle size, and low efficiency. In order to address these difficulties, we successfully produced SNA15, a highly effective AgNPs recombinant protein, was produced after molecular modification of NA33. SNA15 demonstrated good stability, with a yield of 87 mg SNA15 isolated from per liter of Escherichia coli fermentation broth. No apparent precipitation was observed after 7–5 days longer than the original protein—of storage in a 4 ∘ C refrigerator. The generated AgNPs by SNA15, had particle sizes ranging from 3 nm to 20 nm, which were smaller than the original synthesized AgNPs of 30–50 nm by NA33 and demonstrated spectrum antibacterial activity. There will be many opportunities for application in the medical and healthcare industries. The "hypothetical protein" was successfully expressed in Escherichia coli by heterologous expression, and the protein was named NA33. Molecular modification was used to explore the structural domain of NA33 involved in nanosilver synthesis, and the modified protein was named SNA15. SNA15 has a cubic structure, good crystallinity, spherical and subspherical morphology, high vibronic density, small particle size, small specific surface area, good mobility, low agglomeration, high stability, good long-term stability, and spectral inhibition ability.
- Subjects
SYNTHETIC proteins; RECOMBINANT proteins; ESCHERICHIA coli; ANTIBACTERIAL agents; SURFACE area
- Publication
NANO, 2023, Vol 18, Issue 11, p1
- ISSN
1793-2920
- Publication type
Article
- DOI
10.1142/S1793292023500844