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- Title
Suppressive Effects of PO41-14-42-K1, a Component of Pueraria Lobata, on Expression of TGF-beta2 in Human Lens Epithelial Cells Cultured Under High Glucose and on Lens Opacity in Organ Culture.
- Authors
Young Sook Kim; Nan Hee Kim; Dong Ho Jung; Jong Min Kim; Jin Sook Kim
- Abstract
Pueraria lobata has been reported to have comprehensive pharmacological actions in treatment of diabetes and cardiovascular diseases. There is increasing evidence implicating transforming growth factor-beta2 (TGF-β2) in diabetic pathological states of the lens. We hypothesized that PO41-14-42-K1, a component of Pueraria lobata, may involved in the prevention of diabetic eye disease development. We have recently shown the inhibitory effect of PO41-14-42-K1 on advanced glycation end products (AGEs) formation in-vitro. We have examined, high glucose (HG) induced TGF-β2 expression in human lens epithelial (HLE-B3) cells. However, we have also shown, this HG induced TGF-β2 expression was inhibited by PO41-14-42-K1 both at mRNA and its protein level. The aim of the current study is to examine whether, PO41-14-42-K1 is capable of preventing lens opacity in ex-vivo similar to that seen in cataracts. PO41-14-42-K1 treatment (up to 5µg/ml) of HLE-B3 cells led to no significant effect on cell growth by MTT assay. Culturing HLE-B3 cells with 25 mM glucose (HG) induced (2-3 fold increase in) both TGF-β2 mRNA and it proteins levels (p<0.05). This HG induced increase in TGFβ2 expression in HLE-B3 cells was dose dependently inhibited by pre-treating PO41-14-42-K1 (1, 5 µg/mL, p<0.001 vs. HG). In organ culture experiment, PO41-14-42-K1 (1, 5 µg/mL) treatment inhibits, xylose-induced lenses opacity in a dose dependent manner (p<0.001 vs xylose-induced lenses, respectively) suggesting PO41-14-42-K1 may mediate, at least by part, the inhibition of formation of diabetic cataract. These findings show for the first time that PO41-14-42-K1 might be a potential drug for preventing diabetic cataract.
- Subjects
PUERARIA; TRANSFORMING growth factors-beta; EPITHELIAL cells; DIABETES complications; CRYSTALLINE lens diseases; EYE diseases; THERAPEUTICS
- Publication
Diabetes, 2007, Vol 56, pA154
- ISSN
0012-1797
- Publication type
Article