We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti- Pseudomonas aeruginosa Infection in Zebrafish.
- Authors
Chen, Yan-Chao; Qiu, Wanlei; Zhang, Weibin; Zhang, Jingrong; Chen, Roushi; Chen, Fangyi; Wang, Ke-Jian
- Abstract
New antimicrobial agents are urgently needed to address the increasing emergence and dissemination of multidrug-resistant bacteria. In the study, a chemically synthesized truncated peptide containing 22-amino acids derived from a C-type lectin homolog SpCTL6 of Scylla paramamosain was screened and found to exhibit broad-spectrum antimicrobial activity, indicating that it is an antimicrobial peptide (AMP), named Sp-LECin. Sp-LECin possessed the basic characteristics of most cationic AMPs, such as positive charge (+4) and a relatively high hydrophobicity (45%). After treatment with Sp-LECin, the disruption of microbial membrane integrity and even leakage of cellular contents was observed by scanning electron microscopy (SEM). In addition, Sp-LECin could bind lipopolysaccharide (LPS), increase the outer and inner membrane permeability and induce reactive oxygen species (ROS) production, ultimately leading to the death of Pseudomonas aeruginosa. Furthermore, Sp-LECin exhibited potent anti-biofilm activity against P. aeruginosa during both biofilm formation and maturation. Notably, Sp-LECin had no obvious cytotoxicity and could greatly improve the survival of P. aeruginosa-infected zebrafish, by approximately 40% over the control group after 72 h of treatment. This study indicated that Sp-LECin is a promising antibacterial agent with the potential to be used against devastating global pathogen infections such as P. aeruginosa.
- Subjects
ANTIMICROBIAL peptides; PSEUDOMONAS aeruginosa infections; ANTI-infective agents; CATHELICIDINS; PSEUDOMONAS aeruginosa; BRACHYDANIO; MEMBRANE permeability (Biology); PSEUDOMONAS
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 1, p267
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24010267