We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sera Protein Signatures of Endometrial Cancer Lymph Node Metastases.
- Authors
Benbrook, Doris Mangiaracina; Hocker, James Randolph Sanders; Moxley, Katherine Marie; Hanas, Jay S.
- Abstract
The presence of lymph node metastases in endometrial cancer patients is a critical factor guiding treatment decisions; however, surgical and imaging methods for their detection are limited by morbidity and inaccuracy. To determine if sera can predict the presence of positive lymph nodes, sera collected from endometrial cancer patients with or without lymph node metastases, and benign gynecology surgical patients (N = 20 per group) were subjected to electron spray ionization mass spectrometry (ES-MS). Peaks that were significantly different among the groups were evaluated by leave one out cross validation (LOOCV) for their ability to differentiation between the groups. Proteins in the peaks were identified by MS/MS of five specimens in each group. Ingenuity Pathway Analysis was used to predict pathways regulated by the protein profiles. LOOCV of sera protein discriminated between each of the group comparisons and predicted positive lymph nodes. Pathways implicated in metastases included loss of PTEN activation and PI3K, AKT and PKA activation, leading to calcium signaling, oxidative phosphorylation and estrogen receptor-induced transcription, leading to platelet activation, epithelial-to-mesenchymal transition and senescence. Upstream activators implicated in these events included neurostimulation and inflammation, activation of G-Protein-Coupled Receptor Gβγ, loss of HER-2 activation and upregulation of the insulin receptor.
- Subjects
LYMPHATIC metastasis; LYMPH node cancer; ENDOMETRIAL cancer; EPITHELIAL-mesenchymal transition; ELECTRON impact ionization; BLOOD proteins; ESTROGEN receptors; THROMBIN receptors
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 6, p3277
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23063277