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- Title
High-level expression of an acetaldehyde dehydrogenase from Lactiplantibacillus plantarum and preliminary evaluation of its potential as a functional food additive.
- Authors
Zhe Wang; Jian-Ju He; Xin-Xin Liu; Hong-Ling Shi; Yun-Feng Lu; Jun-Ye Shi; Yun-Chao Kan; Lun-Guang Yao; Cun-Duo Tang
- Abstract
Background: Acetaldehyde dehydrogenase (ALDH) is a common oxidoreductase and one of the aldehyde dehydrogenases, which can convert toxic acetaldehyde to harmless acetyl CoA. Results: A novel acetaldehyde dehydrogenase (LpALDH) from Lactiplantibacillus plantarum was obtained by gene mining and then was analyzed by a series of bioinformatics software. Phylogenetic tree results showed that LpALDH was highly homologous with acetaldehyde dehydrogenase derived from Pediococcus sp., and their sequence similarity was 62.9%. Moreover, it was successfully expressed in Escherichia coli BL21, its expression level was 195 U/mL, which was about 600 times than that expressed in L. plantarum. After purification by affinity chromatography, the specific activity of reLpALDH was 1709 U/mg. Its temperature optimum was 35°C, and the optimal reaction pH was 8.0. Moreover, the purified LpALDH showed good stability in simulated gastrointestinal fluids, which indicated that it had great potential in functional food additive field. Conclusions: This research also laid a solid foundation for further molecular modification, optimal expression host selection and application of acetaldehyde dehydrogenases.
- Subjects
FOOD additives; ACETALDEHYDE; FUNCTIONAL foods; ALDEHYDE dehydrogenase; BIOINFORMATICS software; MOLYBDENUM enzymes; ACETYLCOENZYME A; GLUCOSE-6-phosphate dehydrogenase
- Publication
Electronic Journal of Biotechnology, 2023, Vol 63, p1
- ISSN
0717-3458
- Publication type
Article
- DOI
10.1016/j.ejbt.2022.12.005