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- Title
Effect of Antifreeze Protein on Mouse Ovarian Tissue Cryopreservation and Transplantation.
- Authors
Jung Ryeol Lee; Hye Won Youm; Hee Jun Lee; Byung Chul Jee; Chang Suk Suh; Seok Hyun Kim
- Abstract
Purpose: To investigate the effect of antifreeze protein (AFP) supplementation on ovarian vitrification and transplantation. Materials and Methods: In this experimental study, we researched a total of 182 ovaries from 4-week-old ICR mice. The equilibration solution included 20% ethylene glycol (EG), and the vitrification solution included 40% EG, 18% Ficoll, and 0.3 M sucrose. Intact ovaries were first suspended in 1 mL of equilibration solution for 10 min, and then mixed with 0.5 mL of vitrification solution for 5 min. Ovaries were randomly assigned to 3 groups and 0, 5, or 20 mg/mL of type III AFP was added into the vitrification solution (control, AFP5, and AFP20 groups, respectively). The vitrified ovaries were evaluated after warming and 2 weeks after autotransplantation. The main outcome measurements are follicular morphology and apoptosis assessed by histology and the TUNEL assay. Results: A significantly higher intact follicle ratio was shown in the AFP treated groups (control, 28.9%; AFP5, 42.3%; and AFP20, 44.7%). The rate of apoptotic follicles was significantly lower in the AFP treated groups (control, 26.6%; AFP5, 18.7%; and AFP20, 12.6%). After transplantation of the vitrified-warmed ovaries, a significantly higher intact follicle ratio was shown in the AFP20 group. The rate of apoptotic follicles was similar among the groups. Conclusion: The results of the present study suggest that supplementing AFP in the vitrification solution has beneficial effects on the survival of ovarian tissue during cryopreservation and transplantation.
- Subjects
ANTIFREEZE proteins; LABORATORY mice; OVARIAN transplantation; CRYOPRESERVATION of organs, tissues, etc.; ETHYLENE glycol; FERTILITY preservation
- Publication
Yonsei Medical Journal, 2015, Vol 56, Issue 3, p778
- ISSN
0513-5796
- Publication type
Article
- DOI
10.3349/ymj.2015.56.3.778