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- Title
Correlative dynamic contrast MRI and microscopic assessments of tumor vascularity in RIP-tag2 transgenic mice.
- Authors
Sennino, Barbara; Raatschen, Hans-Juergen; Wendland, Michael F.; Fu, Yanjun; You, Weon-Kyoo; Shames, David M.; McDonald, Donald M.; Brasch, Robert C.
- Abstract
The purpose of this study was to define the feasibility of dynamic contrast-enhanced magnetic resonance imaging (MRI) to estimate the vascular density and leakiness of spontaneous islet cell tumors in RIP-Tag2 transgenic mice. Dynamic T1-weighted spoiled gradient echo (SPGR) imaging at 2.0 T was performed in 17 RIP-Tag2 mice using a prototype blood pool macromolecular contrast medium (MMCM), albumin-(Gd-DTPA)35. Kinetic analysis of the dynamic enhancement responses based on a two-compartment model was used to estimate fractional plasma volume (fPV) and the coefficient of endothelial permeability (KPS) for each tumor. The MRI estimate of fPV was correlated on a tumor-by-tumor basis with corresponding microscopic measurements of vascular density. The fPV assays by MMCM-enhanced imaging ranged from 2.4%-14.1% of tissue volume. Individual tumor fPV values correlated significantly ( r = 0.79, P < 0.001) with the corresponding microscopic estimates of vascularity consisting of the combined area densities of lectin-perfused microvessels plus erythrocyte-stained blood lakes. A biotinylated derivative of the albumin-based MMCM confirmed extravasation of the contrast agent from some tumor blood vessels and accumulation in 25% of blood lakes. The KPS values ranged from 0 (no detectable leak) to 0.356 mL/min/100 cm3. Dynamic MMCM-enhanced MRI is feasible in RIP-Tag2 pancreatic tumors, yielding estimates of vascular permeability and microscopically validated measurements of vascular richness. Magn Reson Med, 2009. © 2009 Wiley-Liss, Inc.
- Publication
Magnetic Resonance in Medicine, 2009, Vol 62, Issue 3, p616
- ISSN
0740-3194
- Publication type
Article
- DOI
10.1002/mrm.22040