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- Title
Effects of voluntary wheel running and amino acid supplementation on skeletal muscle of mice.
- Authors
Pellegrino, Maria Antonietta; Brocca, Lorenza; Dioguardi, Francesco Saverio; Bottinelli, Roberto; D'Antona, Giuseppe
- Abstract
The aims of the present study were as follows: (1) to examine the adaptational changes to chronic endurance voluntary exercise and (2) to investigate the effects of amino acid supplementation on the adaptational changes induced by endurance training in hindlimb (gastrocnemius, tibialis, soleus) and respiratory (diaphragm) muscles of mice. Male C57Bl6 mice were divided in four groups: control sedentary, sedentary supplemented with amino acid mixture (BigOne, 1.5 mg g day(-1) in drinking water for 8 weeks), running (free access to running wheels for 8 weeks), and running supplemented with amino acid mixture. Myosin heavy chain (MHC) isoform distribution was determined in all muscles considered. Fiber cross-sectional area (CSA) was measured in the soleus muscle. In all muscles except the tibialis, endurance training was associated with an overall shift towards the expression of slower MHC isoforms. Amino acid supplementation produced a shift towards the expression of faster MHC isoforms in the soleus and diaphragm muscles, and partially antagonized the effects of training. Immunohistochemical analysis of CSA of individual muscle fibers from the soleus muscle suggests that voluntary running produced a decrease in the size of type 1 fibers, and amino acid supplementation during training resulted in an increase in size in both type 1 and type 2A fibers. Collectively, these results suggest that the endurance adaptations induced by voluntary running depend on the muscle type, and that amino acid supplementation is able to modulate both fiber size and MHC isoform composition of skeletal muscles in sedentary and exercised mice.
- Subjects
AMINO acid metabolism; SKELETAL muscle physiology; PHYSIOLOGICAL adaptation; AMINO acids; ANIMAL experimentation; ANIMALS; IMMUNOHISTOCHEMISTRY; MICE; MUSCLE proteins; MUSCLES; PHYSICAL fitness
- Publication
European Journal of Applied Physiology, 2005, Vol 94, Issue 1/2, p655
- ISSN
1439-6319
- Publication type
journal article
- DOI
10.1007/s00421-004-1237-8