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- Title
Evaluation of radiotherapy effect in resveratrol-treated medulloblastoma cancer stem-like cells.
- Authors
Lu, Kai-Hsi; Chen, Yi-Wei; Tsai, Ping-Hsing; Tsai, Ming-Long; Lee, Yi-Yen; Chiang, Chih-Yao; Kao, Chung-Lan; Chiou, Shih-Hwa; Ku, Hung-Hai; Lin, Chi-Hung; Chen, Yann-Jang
- Abstract
Resveratrol (RV), a natural polyphenol derived from red wine, recently showed the potential of anticancer and radiosensitizing effects. A recent study has suggested that the cancer stem cells (CSCs) may reflect the clinical refractory malignancy of brain tumors, including medulloblastoma (MB). The aim of the present study is to investigate the possible role of RV in radiosensitivity of MB cells and MB-associated CSCs. MB-associated CSCs were isolated and cultured by serum-free medium with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). The parental MB cells and MB-CSCs were treated with RV in different concentrations and assessed for cell viability. The treatment includes RV alone, radiation alone, or radiation combined with RV. MB-CSCs selected by serum-free medium with bFGF and EGF can form 3D spheroid formation and display enhanced self-renewal and highly co-expressed “stem cell” genes (Oct-4, Nanog, Nestin, and Musashi-1) as well as antiapoptotic genes (Bcl-2 and Bcl-xL). These MB-CSCs showed significant resistance to radiotherapy as compared to the parental MB cells. Importantly, 100 μM RV could effectively inhibit the proliferation of MB-CSCs and significantly enhance the radiosensitivity in RV-treated MB-CSCs. Our data suggest that RV can effectively inhibit the proliferation and tumorigenicity of MB-CSCs and significantly synergistically enhance radiosensitivity in RV-treated MB-CSCs.
- Subjects
RESVERATROL; ANTINEOPLASTIC agents; RADIATION-sensitizing agents; BRAIN tumors; MEDULLOBLASTOMA; SERUM-free culture media; FIBROBLAST growth factors; EPIDERMAL growth factor
- Publication
Child's Nervous System, 2009, Vol 25, Issue 5, p543
- ISSN
0256-7040
- Publication type
Article
- DOI
10.1007/s00381-009-0826-6