We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Digital spatial profiling application in breast cancer: a user's perspective.
- Authors
McCart Reed, Amy E.; Bennett, James; Kutasovic, Jamie R.; Kalaw, Emarene; Ferguson, Kaltin; Yeong, Joe; Simpson, Peter T.; Lakhani, Sunil R.
- Abstract
The disciplines of oncology and pathology are at present experiencing a wave of changes as precision medicine becomes embedded as standard-of-care. Consequently, the need to assess increasing numbers of biomarkers simultaneously has become more urgent and recognising the vast intra-tumoural heterogeneity, including within the microenvironment, requires a complex dimensional understanding of the localisation of the biomarker expression. Digital spatial profiling (DSP; nanoString™) technology spatially resolves and digitally quantifies proteins in a highly multiplexed assay, underpinned by the nCounter® barcoding platform. We present the application of this technology to breast cancer samples. Applying the 'off the shelf' cancer panel and a custom-conjugated E-cadherin antibody, we quantify vast intra-tumoural heterogeneity in immunological and tumour markers, and demonstrate a need for focussed selection of target cell populations. The technology offers enormous potential not only for making research advances but also for improving standard operating procedures in diagnostic applications.
- Subjects
BREAST cancer; CELL populations; STANDARD operating procedure; INDIVIDUALIZED medicine
- Publication
Virchows Archiv: European Journal of Pathology, 2020, Vol 477, Issue 6, p885
- ISSN
0945-6317
- Publication type
Article
- DOI
10.1007/s00428-020-02821-9