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- Title
A critical analysis of three quantitative methods of assessment of hepatic steatosis in liver biopsies.
- Authors
Catta-Preta, Mariana; Mendonca, Leonardo; Fraulob-Aquino, Julio; Aguila, Marcia; Mandarim-de-Lacerda, Carlos; Mendonca, Leonardo Souza; Aguila, Marcia Barbosa; Mandarim-de-Lacerda, Carlos Alberto
- Abstract
The issue of adequately quantitatively evaluating hepatic steatosis is still unresolved. Therefore, we compared three methods of quantitative assessment. Two groups of mice (n = 10 each) were fed standard chow (10% fat, SC group) or a high-fat diet (60% fat, HF group) for 16 weeks, and hepatic triglyceride (HT) and liver tissue were then studied. Paraplast-embedded tissues stained by hematoxylin and eosin (H-E) were compared to frozen sections stained by Oil Red-O (ORO). In addition, the volume density of steatosis (Vv[steatosis, liver]) was measured by point counting (P-C, sections H-E or ORO) or by image analysis (I-A, sections ORO). HT was significantly higher in the HF group (104% greater, P = 0.0004) than in the SC group. With P-C and H-E, Vv[steatosis, liver] was 4.80 ± 0.90% in the SC group and 33.50 ± 3.17% in the HF group (600% greater, P < 0.0001). With P-C and ORO, Vv[steatosis, liver] was 4.86 ± 0.89% in the SC group and 25.21 ± 1.27% in the HF group (420% greater, P < 0.0001). With I-A and ORO, Vv[steatosis, liver] was 4.17 ± 0.85% in the SC group and 23.35 ± 1.58% in the HF group (460% greater, P < 0.0001). Correlations between Vv[steatosis, liver] and HT were strong and significant in all methods. In conclusion, all methods were appropriate and reproducible. In P-C and H-E, there is a slight overestimation of steatosis in the HF animals in comparison to frozen sections and ORO; in frozen sections, differences between P-C and I-A are insignificant.
- Subjects
FATTY degeneration; FATTY liver; LIVER diseases; BIOPSY; STEREOLOGY; ANALYSIS of triglycerides; ANIMAL experimentation; COMPARATIVE studies; LIVER; RESEARCH methodology; MEDICAL cooperation; MICE; REGRESSION analysis; RESEARCH; EVALUATION research; RESEARCH bias
- Publication
Virchows Archiv: European Journal of Pathology, 2011, Vol 459, Issue 5, p477
- ISSN
0945-6317
- Publication type
journal article
- DOI
10.1007/s00428-011-1147-1