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- Title
Effects of sample handling on the stability of interleukin-6 in patients with breast neoplasms.
- Authors
Wang, Yunxiu; Wang, Baiye; She, Lanhui; Wang, Jijuan; Chen, Yingting; Huang, Di; Han, Guang; Lu, Miaolian; Wu, Xiaobin; Wan, Zemin; He, Min; Ke, Peifeng; Huang, Xianzhang; Liu, Hongcan
- Abstract
To investigate the influence of preservation methods and processes on the plasma interleukin-6 (IL-6) stability. Lithium-heparin plasma was collected from female patients: 32 female patients with invasive breast neoplasms and 20 healthy females. Each sample was divided into three tubes. Samples were stored at different temperatures or at different times. The concentration of IL-6 was detected. IL-6 levels in patients were not altered significantly compared to the control group after storage at 4 °C or 25 °C for 12 h. However, IL-6 levels were significantly higher compared to controls (p<0.05) after storage at 25 °C for 48 h. IL-6 levels in patients with breast neoplasms were significantly higher compared to the control group (p<0.05) when stored at 4 °C after 12 h. IL-6 levels in patients with breast neoplasm increased more than 10-folds compared to the control group after only 2 h storage at 25 °C. Concentrations of IL-6 in breast neoplasms samples significantly change under different storage conditions. Pretreatment needs to be standardized for blood sample handling procedure. Comparison of different storage conditions of IL-6 levels may not be reliable.
- Subjects
STATISTICAL reliability; TEMPERATURE; INTERLEUKIN-1; BLOOD collection; CANCER patients; COMPARATIVE studies; DESCRIPTIVE statistics; COLLECTION &; preservation of biological specimens; BREAST tumors
- Publication
Journal of Laboratory Medicine, 2022, Vol 46, Issue 2, p115
- ISSN
2567-9430
- Publication type
Article
- DOI
10.1515/labmed-2020-0155