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- Title
COMPUTATIONAL ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL PROPERTIES OF GONADOTROPIN RELEASING HORMONE RECEPTOR ISOFORMS OF ZEBRAFISH.
- Authors
Kumar, Gulshan; Devanand, T. N.; Leya, Tasok; Swain, Sambid; Prasad, K. S. Krishna
- Abstract
Reproduction in teleost is controlled by brain-hypophyseal-gonadal axis as like in tetrapoda. In this system GnRH secreted from hypothalamus binds to GnRH receptors in pituitary and induces secretion of gonadotropin hormones. These hormones induce gonadal maturation and spawning in fish. The study of this system is important for developing induce breeding methodology as well as understanding the physiology of fish. Zebrafish (Danio rario) is an important model organism for research for both human and fish. It is also an interesting animal as it is having multiple isoforms of GnRH and its receptors. Sequence information, tissue distribution and signalling responses of all the four receptors (zfGnRH-R) is available and predicted some sort of sub-functionalization. The present study was designed to analyse physic-chemical properties, secondary structure, tertiary structure and membrane orientation properties of four zfGnRH-Rs using various bioinformatics tools. All four receptors showed variation in sequence length and their molecular weights. All contain Leu as most abundant amino acid. They all are positively charged, hydrophobic and basic proteins. They are composed of mainly α-helix and random coils. They all have 7 TM helices as a like other GPCRs. Random coils are dominant between these TM helices. Some of the loops present between the TM helices showed variation in the length and have tendency to form α-helix and β-sheet. C-terminal helix remains perpendicular to 7th TM helix. All the four receptors require around 31 Å thick lipidmembrane and have negative membrane transfer energy. They remain slightly tilted in the membrane.
- Subjects
GONADOTROPIN releasing hormone; LUTEINIZING hormone releasing hormone receptors; BASIC proteins; BRACHYDANIO; FISH physiology; HYPOTHALAMUS; HORMONE receptors
- Publication
Journal of Experimental Zoology India, 2020, Vol 23, Issue 2, p1841
- ISSN
0972-0030
- Publication type
Article