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- Title
Complete degradation of di-n-butyl phthalate by Glutamicibacter sp. strain 0426 with a novel pathway.
- Authors
Ren, Chongyang; Wang, Yiying; Wu, Yanan; Zhao, He-Ping; Li, Li
- Abstract
Di-n-butyl phthalate (DBP) is widely used as plasticizer that has potential carcinogenic, teratogenic, and endocrine effects. In the present study, an efficient DBP-degrading bacterial strain 0426 was isolated and identified as a Glutamicibacter sp. strain 0426. It can utilize DBP as the sole source of carbon and energy and completely degraded 300 mg/L of DBP within 12 h. The optimal conditions (pH 6.9 and 31.7 °C) for DBP degradation were determined by response surface methodology and DBP degradation well fitted with the first-order kinetics. Bioaugmentation of contaminated soil with strain 0426 enhanced DBP (1 mg/g soil) degradation, indicating the application potential of strain 0426 for environment DBP removal. Strain 0426 harbors a distinctive DBP hydrolysis mechanism with two parallel benzoate metabolic pathways, which may account for the remarkable performance of DBP degradation. Sequences alignment has shown that an alpha/beta fold hydrolase (WP_083586847.1) contained a conserved catalytic triad and pentapeptide motif (GX1SX2G), of which function is similar to phthalic acid ester (PAEs) hydrolases and lipases that can efficiently catalyze hydrolysis of water-insoluble substrates. Furthermore, phthalic acid was converted to benzoate by decarboxylation, which entered into two different pathways: one is the protocatechuic acid pathway under the role of pca cluster, and the other is the catechol pathway. This study demonstrates a novel DBP degradation pathway, which broadens our understanding of the mechanisms of PAE biodegradation.
- Subjects
RESPONSE surfaces (Statistics); ENDOCRINE glands; PHTHALIC acid; SOIL pollution; SEQUENCE alignment; BIOREMEDIATION; PHTHALATE esters; CATECHOL
- Publication
Biodegradation, 2024, Vol 35, Issue 1, p87
- ISSN
0923-9820
- Publication type
Article
- DOI
10.1007/s10532-023-10032-7