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- Title
Laser-synthesized nanocrystalline, ferroelectric, bioactive BaTiO<sub>3</sub>/Pt/FS for bone implants.
- Authors
Jelínek, Miroslav; Buixaderas, Elena; Drahokoupil, Jan; Kocourek, Tomás; Remsa, Jan; Vanék, PPemysl; Vandrovcová, Marta; Doubkova, Martina; Bacáková, Lucie
- Abstract
The goal of our study is to design BaTiO[sub 3] ferroelectric layers that will cover metal implants and provide improved osseointegration. We synthesized ferroelectric BaTiO[sub 3] layers on Pt/fused silica substrates, and we studied their physical and bio-properties. BaTiO[sub 3] and Pt layers were prepared using KrF excimer laser ablation at substrate temperature T[sub s] in the range from 200°C to 750°C in vacuum or under oxygen pressure of 10 Pa, 15 Pa, and 20 Pa. The BaTiO[sub 3]/Pt and Pt layers adhered well to the substrates. BaTiO[sub 3] films of crystallite size 60-140 nm were fabricated. Ferroelectric loops were measured and ferroelectricity was also confirmed using Raman scattering measurements. Results of atomic force microscopy topology and the X-ray diffraction structure of the BaTiO[sub 3]/Pt/fused silica multilayers are presented. The adhesion, viability, growth, and osteogenic differentiation of human osteoblast-like Saos-2 cells were also studied. On days 1, 3, and 7 after seeding, the lowest cell numbers were found on non-ferroelectric BaTiO[sub 3], while the values on ferroelectric BaTiO[sub 3], on non-annealed and annealed Pt interlayers, and on the control tissue culture polystyrene dishes and microscopic glass slides were similar, and were usually significantly higher than on non-ferroelectric BaTiO[sub 3]. A similar trend was observed for the intensity of the fluorescence of alkaline phosphatase, a medium-term marker of osteogenic differentiation, and of osteocalcin, a late marker of osteogenic differentiation. At the same time, the cell viability, tested on day 1 after seeding, was very high on all tested samples, reaching 93-99%. Ferroelectric BaTiO[sub 3] films deposited on metallic bone implants through a Pt interlayer can therefore markedly improve the osseointegration of these implants in comparison with non-ferroelectric BaTiO[sub 3] films.
- Subjects
BONE surgery; NANOCRYSTAL synthesis; FERROELECTRIC materials; BIOACTIVE compounds; BARIUM titanate; PLATINUM
- Publication
Journal of Biomaterials Applications, 2018, Vol 32, Issue 10, p1464
- ISSN
0885-3282
- Publication type
Article
- DOI
10.1177/0885328218768646