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- Title
INFLUENCE OF RHIZOPLANE AND RHIZOSPHERE BACTERIA ISOLATED FROM INFECTED PLANTS OF WITHANIA SOMNIFERA (DUNAL) IN BIOTRANSFORMATION OF WITHANOLIDES AND STEROLS.
- Authors
Madhusudan, S.; Dakshayini, P. N.; Bhushanam, M.; Mathias, Arun Jyothi
- Abstract
Endophytes are a group of endosymbiotic microorganisms, which colonizes the plants and they can be readily isolated in the laboratory on a specific microbial medium. These endophytes are known to be the reservoirs of many novels, secondary metabolites. Endophytic microorganisms, especially bacteria are known to colonize many plants, including medicinal plants. Apart from synthesizing the secondary metabolites in the associated plants, the endophytic microorganisms are known to increase the ability of synthesizing the bioactive components. In the current study, we investigated on analyzing these endophytic microorganisms especially bacteria associated with the medicinal plant that can synthesize the secondary metabolite compounds under abiotic stress in Withania somnifera. Entophytic bacteria from rizzoplane and risosphere was isolated from the leaves and roads of W. somnifera. Pure bacterial isolates were identified using 16S rRNA sequencing. 16S rRNA sequences showed that the bacterial isolates were found to be in close resemblance with Bacillus velezensis, Bacillus amyloliquefaciens and Pseudomonas floridensis. Further, the pure culture cell free extracts with Withanolides and sterols substrates were extracted and analyzed using HPLC and TLC. Out of the total bacterial cultures isolated, B9 and B6 isolates showed significant bands when compared with control. Similarly, B1 isolates showed unique bands when compared with control. The rise in the production of the secondary metabolite components such as withaferin A, withanolide D was significant as seen when treated with bacterial endophytes than the control. The findings of the current research can be used in tracing the application of potent bacterial endophytes in modifying the natural chemical structure of the secondary metabolite compounds.
- Subjects
RHIZOBACTERIA; WITHANIA somnifera; ENDOPHYTIC bacteria; WITHANOLIDES; STEROLS; METABOLITES
- Publication
Biochemical & Cellular Archives, 2023, Vol 23, Issue 2, p1235
- ISSN
0972-5075
- Publication type
Article
- DOI
10.51470/bca.2023.23.2.1235