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- Title
ASSESSMENT OF URINARY 8-HYDROXYDEOXYGUANOSINE IN DIABETIC PATIENTS AS A BIOMARKER OF OXIDATIVE DNA DAMAGE.
- Authors
Saleem, Hadeel Fadhil; Ali Shafi, Farha A.
- Abstract
Diabetes mellitus disease (DMD) is the most rapidly growing chronic metabolic disease in the world. Recent evidence reveals that high blood glucose is associated with cancer risk in diabetic patients. In diabetes type 2 patients, based on HbA1c-levels and another factor to predict possible cancer risk. The current study aims to analyze DNA oxidation biomarkers by measuring 8-hydroxy-22-deoxyguanosine (8-OHdG). A total of 119 patients previously diagnosed with type 2 diabetes (53 male and 66 female) were enrolled in this study from a local diabetes clinic (Baghdad). The control group included 57 nondiabetic healthy volunteers (26 male, 31 females). BMI was calculated as weight (W) in kilograms divided by the square of height (H) in meters) and four to five milliliters of venous blood were withdrawn from each participant (patients and control) via venipuncture under sterile conditions. Glycated hemoglobin was measured by using spinreact kits (Spin). Urine samples were collected from each participant (patients and controls) to analyze The 8-OHdG concentrations in urine using 8-OHdG (8- Hydroxydeoxyguanosine) ELISA Kit. The HbA1c value in patients was significantly higher than those of control (P<0.0001). The 8-OHdG value in urine samples of diabetic patients was higher than in the control group. However, the differences between groups (patients and control) were statistically not significant (P>0.05). The current study’s finding does not approve the hypothesis that oxidative stress and its subsequent genomic damage are growing in diabetics patients. Also, the present study does not express any significant associations between HbA1c value and oxidative markers(8-OHdG ). Most previous studies matched healthy individuals to type 2 diabetes patients and could identify induced oxidative damage in type 2 diabetes patients.
- Subjects
TYPE 2 diabetes; METABOLIC disorders; DNA damage; BIOMARKERS; DEOXYGUANOSINE
- Publication
Biochemical & Cellular Archives, 2021, Vol 21, Issue 2, p3107
- ISSN
0972-5075
- Publication type
Article