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- Title
EVALUATION OF HYDROGEN PEROXIDE ON CONTROLLING SAPROLEGNIASIS IN COMMON CARP, CYPRINUS CARPIO L.
- Authors
Ashour, Ahmad A.; Salman, Noor M.; Mustafa, Sanaa A.; Nemah, Rula O.
- Abstract
This study was aimed to investigate the efficacy of hydrogen peroxide on controlling mortality associated with Saprolegniasis in common carp, Cyprinus carpio L. Saprolegniasis was experimentally induced in common carp at concentration of 2×104 zoospore/L. A total of 100 fish were randomly divided into five groups (fish/treatment) control negative group, abraded but not challenged, infected group, challenged with Saprolegnia spp. The 2nd, 3rd, 4th and 5th groups were challenged with Saprolegnia spp. and therapeutic hydrogen peroxide bath treatments of 13 and 6 mL/L for 30 min addition to formalin bath of 0.15ml/L for 60 min were administered respectively for 3 days interval. Hydrogen peroxide treatments of 6 mL/L were harmful (relative to lower concentrations) to test fish and resulted in 35% mortality. Fish groups treated with H2O2 at concentrations of 1 and 3 ml/L reported the lowest mortality rate reached up to 10, 25% respectively, followed by formalin treated group reached up to 20%. Mortality rate in untreated positive control reached up to 80%. After 15 days from the experimental period, albumin level in T1, T2, T3 and T4 registered significant increase (p<0.05) compared to C+C- groups. While, globulin and total protein showed significant increase in T1 and T4 relative to C+ group and there was no significant difference between T2 and T3 groups. At the end of the experimental period there was significant increase (p<0.05) in ALP activity in all treatment groups were over the C- and C+ groups. However, there was no significant differences (p>0.05) among all treatment groups. Although, the use of H2O2 did not completely reduce fungal infection, but it may diminish the frequency of the use of hazardous chemicals such as malachite green. However, search for alternatives therapies must continue and it is of vital importance for the development of effective treatment against Saprolegniasis in fish.
- Subjects
HYDROGEN peroxide; CARP; FUNGAL diseases of fish; FISH mortality; SAPROLEGNIA
- Publication
Biochemical & Cellular Archives, 2019, Vol 19, Issue 2, p4247
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2019.19.2.4247